The effect of pulsed electromagnetic fields for in vitro wound healing exposure systems

THE EFFECT OF PULSED ELECTROMAGNETIC FIELDS FOR IN VITRO WOUND HEALING EXPOSURE SYSTEMS
SUMMARY
Electromagnetic fields have achieved important role as stimulator and therapeutic facility in biology and medicine (Blank & Findl, 2013). The effects of low magnitude, low frequency pulsed electromagnetic fields have been a period of study since past few decades, which was mainly on the treating soft tissue injuries, skin ulcers, nonuniform bone fractures and degenerative nerve healing (Hug & Röösli, 2012b). Chronic wounds are a major healthcare problem for patient morbidity and contribute significantly to the cost of health care in the world. Chronic ulcers or wounds are breaks in the skin of greater than 6 weeks or with frequent recurrence. The most common etiologies of chronic ulcers include venous leg ulcers, pressure ulcers (PrUs), diabetic neuropathic foot ulcers (DFUs), and leg ulcers of arterial insufficiency (Markova & Mostow, 2012). From this point of view, the main purpose of the present study is to investigate the effects of pulsed radio frequency energy (PRFE) at 27.12 MHz carrier frequency on the proliferation and migration of cells involved in wound healing in vitro with the ultimate aim of developing an applicator antenna. One of the aims of this study was to investigate effect of Pulsed electromagnetic field (PEMF) at 75 Hz frequency by applying on the same cell lines and investigated its effects on wound healing. The applications of the developed PRFE and PEMF exposure systems have been investigated in terms of the experimental evaluation of in vitro Scratch Assay, MTT Assay, and Immunohistochemistry analyses. The PRFE antennas and PEMF coils were designed and simulated using CST Microwave studio software and measurements were carried out to verify the results. E field and H field
simulations were also carried out with biological materials (tissue or cell culture in petri dishes). Through this study, we experimentally proved that it is possible to optimize PRFE and PEMF parameters (frequency, f, and magnetic flux density, B ) of the applied irradiation for accelerated wound healing. Obtained results showed that the application of PEMF and PRFE increase proliferation of non-cancerous cells while cease the proliferation of cancer cells stopped.