Custom designed optical tweezer for trapping yeast cells

  • Eser Sahibi Çobanoğlu, Nur
  • Tez Danışmanı Aziz KOLKIRAN
  • Tür Yüksek Lisans
  • Yayın Tarihi 2018
  • Yayıncı İzmir Katip Çelebi Üniversitesi Fen Bilimleri Enstitüsü
  • Tek Biçim Adres Http://hdl.handle.net/11469/375

The goal of this study is developing custom designed optical tweezer which uses two
He-Ne lasers (λ=632.8 nm) parallel to each other to trap yeast cells. The
characteristic specs of this optical tweezer determined by Brownian Motion of
polystyrene beads in water. Stiffness and trapping forces of optical tweezer
calculated for laser 1, laser 2 and when both lasers used simultaneously. Laser 2 can
trap and move particle to laser 1 and multiple particles can be trapped by the laser 1.
After determination of specs of optical tweezer, yeast cells in yogurt culture medium
were trapped by laser 1 and laser 2. Stiffness of optical tweezer and trapping force on
yeast cells were determined by drag force and Brownian motion methods by
assuming the medium was homogenous and had viscosity of 0.038 kg/ms. Viscosity
depends on the concentration of fluid and surface. Viscosity of the yogurt culture
medium which was used in experiments was calculated theoretically with respect to
velocity of fluid and specs of optical tweezer calculated previously. Possibility of
application inside yeast cells was studied theoretically by using viscosity of
cytoplasmic medium. As a result of this study, this custom designed optical tweezer
which uses two He-Ne lasers are applicable in life sciences like inside cells, viscosity
measurements and drug delivery systems.

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Detaylı Görünüm
Eser Adı
(dc.title)
Custom designed optical tweezer for trapping yeast cells
Eser Sahibi
(dc.contributor.author)
Çobanoğlu, Nur
Tez Danışmanı
(dc.contributor.advisor)
Aziz KOLKIRAN
Yayıncı
(dc.publisher)
İzmir Katip Çelebi Üniversitesi Fen Bilimleri Enstitüsü
Tür
(dc.type)
Yüksek Lisans
Özet
(dc.description.abstract)
The goal of this study is developing custom designed optical tweezer which uses two He-Ne lasers (λ=632.8 nm) parallel to each other to trap yeast cells. The characteristic specs of this optical tweezer determined by Brownian Motion of polystyrene beads in water. Stiffness and trapping forces of optical tweezer calculated for laser 1, laser 2 and when both lasers used simultaneously. Laser 2 can trap and move particle to laser 1 and multiple particles can be trapped by the laser 1. After determination of specs of optical tweezer, yeast cells in yogurt culture medium were trapped by laser 1 and laser 2. Stiffness of optical tweezer and trapping force on yeast cells were determined by drag force and Brownian motion methods by assuming the medium was homogenous and had viscosity of 0.038 kg/ms. Viscosity depends on the concentration of fluid and surface. Viscosity of the yogurt culture medium which was used in experiments was calculated theoretically with respect to velocity of fluid and specs of optical tweezer calculated previously. Possibility of application inside yeast cells was studied theoretically by using viscosity of cytoplasmic medium. As a result of this study, this custom designed optical tweezer which uses two He-Ne lasers are applicable in life sciences like inside cells, viscosity measurements and drug delivery systems.
Kayıt Giriş Tarihi
(dc.date.accessioned)
2018-08-09T13:23:20Z
Açık Erişim Tarihi
(dc.date.available)
2018-08-09
Yayın Tarihi
(dc.date.issued)
2018
Yayın Dili
(dc.language.iso)
eng
Alternatif Yayın Başlığı
(dc.title.alternative)
Özel yapım optik cımbız ile maya hücresi manipülasyonu
Tek Biçim Adres
(dc.identifier.uri)
Http://hdl.handle.net/11469/375
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