Effect of resveratrol on bone formation in the expanded inter-premaxillary suture: early bone changes

Objective –The aim of this study was to evaluate the effects of local resveratrol
(RSVL) administration on bone formation in response to expansion of the interpremaxillary
suture, in rats.
Material and Methods – Twenty 50- to 60-day-old male Wistar rats were separated
into two equal groups. Both groups were subjected to expansion, and 30 cN of force
was applied to the maxillary incisors with helical-spring. Twenty-four hours after
appliance placement, single-dose 10 lmol ⁄ kg RSVL in the dimethylsulfoxide
(DMSO) was injected to the inter-premaxillary suture in the experimental group. In
the control group, the same amount of DMSO was injected to the suture of rats. Bone
formation in the suture was evaluated histomorphometrically. The area of new bone
(lm2), the perimeter around the new bone (lm), Feret s diameter (lm), the
percentage of new bone to non-ossified tissue (%), and the number of osteoblast
were measured and compared. Mann–Whitney U-test was used for statistical
evaluation at p < 0.05 level.
Results – Statistically significant differences were found between the groups for all
histomorphometric parameters. New bone area (p < 0.001), bone perimeter
(p < 0.001), Feret s diameter (p < 0.001), percentage of new bone (p < 0.001), and
the number of osteoblast (p < 0.001) were significantly larger in the experimental
group when compared with the control. Bone histomorphometric measurements
revealed that bone architecture in the RSVL treated rats was improved.
Conclusions – Local application of RSVL during the early stages to orthopedically
expanded inter-premaxillary suture area may stimulate bone formation and shorten
the retention period, in rats.